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DPPH Test (with calculation of IC50) for evaluation of Antioxidant activity (Bangla Tutorial )
 
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Performed by Mohammad Shah Hafez Kabir Founder and CEO, GUSTO A Research Group B. Pharm, Department of Pharmacy Faculty of Science and Engineering International Islamic University Chittagong (IIUC) Google Scholar: https://scholar.google.com/citations?user=YO6y-jYAAAAJ&hl=en ResearchGate: https://www.researchgate.net/profile/Mohammad_Kabir9
Views: 36936 GUSTO A Research Group
Quantus™ Fluorometer - Sample Measurement
 
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http://www.promega.com/products/pm/quantus-fluorometer/ Simple, accurate, and sensitive: learn about sample measurement using the Quantus™ Fluorometer!
Views: 1111 Promega Corporation
Qubit® 2.0 Fluorometer in Action
 
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To Learn More visit, http://www.lifetechnologies.com/qubit The Qubit® 2.0 Fluorometer quantitates DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity. It is designed for molecular biology labs that work with: • Precious samples that are rare or difficult to process • Applications requiring precise measurement such as real-time PCR The Qubit® 2.0 Fluorometer utilizes specifically designed fluorometric technology using Molecular Probes® dyes. These fluorescent dyes emit signals ONLY when bound to specific target molecules, even in the presence of free nucleotides or degraded nucleic acids. Qubit® fluorometric quantitation provides the most specific and sensitive DNA and RNA quantitation available, even at low concentrations. Transcript Dr. Amr Abid: It's hard to believe how something so simple, so light can deliver such performance. That is exactly what Qubit 2.0 does. Ashleigh Young: Qubit was a real revolution when we launched version 1.0 in 2006. We learned so much from the scientists. We listened to their suggestions and we brought them into version 2.0. With Qubit 2.0, you don't need to change yourself to fit the device. It fits you. Dr. Amr Abid: Qubit 2.0 is simply the most specific and sensitive device amongst all nucleic acid and protein quantification devices. Voice: Much time, effort, and research money is invested to obtain precious nucleic acid and protein samples. We developed Qubit 2.0 because we believe that knowing precisely and accurately the quantity of DNA, RNA or protein is so critical to the success of the downstream applications like sequencing, PCR, RT-PCR, transfection and immunoassays. Dr. Amr Abid: The device provides trust and confidence ensuring downstream applications don't suffer from inaccurate quantification. Voice: It's no surprise that these reagents and devices are now recommended by leading journals and institutions as the best for quantifying RNA or DNA in applications like q-PCR, RT-PCR and sequencing. Qubit 2.0's specificity is due to the use of different fluorophores for DNA, RNA, single-stranded DNA and protein. UV quantification method simply cannot tell or confirm if what you have in the tube is RNA, DNA, nucleotide or a mix. Basic Qubit protocol is simple and shouldn't take more than two minutes. The first step is to prepare the working solution. Use 200 microliters of buffer for each tube. Use 1 microliter of dye for each tube. Because we have five tubes in our DNA quantification assays, we will need 5 microliters of dye and 1 mL of the buffer. Mix the dye and the buffer in 1.5 mL microtubes. Now we're ready to add the standards and the DNA sample. Use thin-wall PCR tubes. For DNA quantification assay, we need two standards. The total volume in each tube should be 200 microliters. Add 190 microliters of working solution prepared previously to each tube. Add 10 microliters of the standard one in the first tube and 10 microliters of standard two in the second tube. For the DNA sample, we can use between 1 to 20 microliters of each DNA solution to an assay tube. In this case, we add 198 microliters of the working solution prepared previously in our three DNA tubes. In each of the three DNA tubes, we add 2 microliters of DNA sample and mix. We incubate the five tubes at room temperature for two minutes. We now have our two standards and three DNA samples. We're ready to measure them in the Qubit 2.0 Fluorometer. On the homescreen, you have a choice for DNA, RNA, or protein quantification. Qubit 2.0 Fluorometer has a touch screen. To choose DNA, just touch the appropriate area. You will have a choice between different ways to quantify DNA: double-stranded DNA in high-sensitive mode, double-stranded DNA in broad range mode or single-stranded DNA. In our case, we will use the broad range mode. Standard screen is automatically displayed. Insert standard number one in the sample chamber and press "Read." When the reading is done, you'll be prompted to insert standard number two and press "Read." Qubit 2.0 Fluorometer is very simple and intuitive. It will guide the user through all the measurement steps. Ashleigh Young: The Qubit 2.0 is not just a new product. It's way more than that and yet thousands and thousands of users will instantly know how to use it. Performance with simplicity. This is the very DNA of Life Technologies. Voice: Qubit fluorometric quantitation: sensitive, specific, affordable. Simply better than [using].
Using Standard Curve to Estimate DNA Quantity - Forensic Focus #4
 
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Submit your questions at http://www.thermofisher.com/forensicfocus Everyone wants to know how much DNA is in their extract, but then they ask: how can I tell if my estimate is accurate? The standard curve holds the answers. A standard curve is a tool that allows us to estimate the DNA concentration of unknown samples by comparing them to standards with known DNA concentrations. In this example, the standards consist of a 10-fold dilution series ranging from 50 ng/ul down to 5 pg/ul. During each PCR cycle, the amount of fluorescent signal for each standard in the dilution seies is measured. When the fluorescent signal crosses the detection threshold the cycle number is recorded as a Ct value, or threshold cycle value. The Ct value is what ultimately is used to create the standard curve. The Ct values are inversely proportional to the concentration of DNA in the standards. The high-concentration, 50 ng/ul standard will cross the detection threshold first, generating a “low” Ct. The low-concentration, 5 pg/ul standard will take many more cycles to cross the same threshold - and therefore the Ct will be higher. The Ct values for each dilution of the standard curve are plotted on a graph, and the software generates a regression line that fits the data. Because the standards are 10-fold dilutions, we expect the change in Ct from one standard to the next to be uniform. An uneven distribution of Ct values might indicate that the dilution series was not accurately pipetted. Let’s take a look at the standard curve for a specific DNA target, the small autosomal target. The X axis is the log of the known standard concentrations. The Y axis is the Ct value of each standard. Now, Do you see the quality metrics at the bottom of the screen? Let’s review Slope, Y intercept, and R2.? The slope measures the efficiency of the PCR reaction. In a perfect world, a slope of -3.3 indicates that the PCR reaction is 100% efficient; the target DNA is doubled each cycle. Two copies become four; four become eight; and so on. The Y intercept is the expected Ct value for a 1ng/ul sample. The R2 value measures how well the regression line fits the data points. A line that fits the data points perfectly has an R2 of 1. If your data points are scattered, the R2 value for the line will be lower. The Ct values of your standards affect the slope, the Y intercept, and the R2 value. It is very important to prepare the standard dilution series carefully to ensure consistent and accurate results! Running the standards in duplicate can help ensure you have a high quality standard curve. Once your standard curve passes the metrics test, it can be used to evaluate an unknown sample! The Ct value of the unknown sample is measured, and compared to the standard curve to estimate the DNA concentration of the unknown sample. Couldn’t be simpler! That’s it for today. If you have other questions, just click on the link below. And don’t forget- when in doubt, refer Back to the Bases!
Office Tutorials - Determining the Concentration of an Unknown Sample (Microsoft Excel 2011)
 
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In this tutorial I show you how to (again) generate a standard curve, and use that standard curve to determine the concentration of an unknown solution (assuming that we are able to obtain its absorbance). I perform this tutorial in Excel 2011 on a Mac. For more office tutorials on multiple operating systems and in different programs, visit my website: http://iws.collin.edu/mgarcia/Office/
Views: 175822 Mark Garcia
Protein Thermal Shift: Introduction (1/4) by Life Technologies
 
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To learn more and download a free trial version of the software, visit: http://www.lifetechnologies.com/proteinmelt This video segment presents an overview of Protein Thermal Shift technology (also known as Differential Scanning Fluorimetry), a quick, easy, and inexpensive approach to studying protein stability and ligand binding under various test conditions using a real-time PCR system. The theory behind Protein Thermal Shift is presented, along with an overview of the workflow.
Custom Assay Development Services by Life Technologies
 
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Learn more at http://www.lifetechnologies.com/custom When an "off-the-shelf" assay does not provide the right fit for your studies, the Custom Biology Services team can craft an assay that helps solve your biological problems and meets your unique specifications, timelines and budget. Choose from custom biochemical, cell-based and TaqMan® protein assays; and collaborate with our Custom Biology team of scientists who are acutely familiar with the full range of Life Technologies assay formats. Then utilize our SelectScreen® services to seamlessly transition into screening your custom-made assay once the assay is developed. You know our customers really are the experts in the biology that they're studying. But as a tool provider, we have an intimate familiarity with the technology that can help our customers solve a biological problem. Some of the services that our custom biology team can offer to our customers include Custom Cellular and Biochemical Assay Development, SRNA Screening, TaqMan® Protein Assay Development as well as Cryopreservation services. Additionally, we can take Cell-based assays or stem cell-based assays and configure not just large scale provisioning these cells but we can transfer them directly into a screening operation and seamlessly move our customers from an assay-development paradigm into more of an operational screening exercise. Working with scientists from other companies is exciting because as scientists, we always want to keep our fingers on the pulse of the research community and working on custom projects is a great way to do that. On a personal level, it's really fulfilling. There's nothing better than hearing a customer say at the end of a project, "We're excited about what you've done. We're excited to get the material in. We're happy with the service and we want to continue this relationship and bring you more work in the future." Learn more at http://www.lifetechnologies.com/custom
Residual DNA Analysis Workflow
 
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Learn more at http://lifetechnologies.com/resdnaseq This video contains Tips and Tricks for residual DNA quantitation using the resDNASEQ® Residual DNA Quantitation System, including PrepSEQ® Express Sample Preparation kits, the AutoMate Express™ Nucleic Acid Extraction System, and the Applied Biosystems® 7500 FAST Real-Time PCR System.
Sweet Science: A New Device for Checking Apple Ripeness
 
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Agriculture and Agri-Food scientists in Summerland, B.C. and Kentville, N.S., are working to give apple producers cutting edge technology to ensure market success. They are developing data protocols for the DA Meter, a special tool that helps fruit producers to determine optimal ripeness of their crop. When it first came on the market, the DA Meter was not well adapted to reading apple ripeness. With the new protocols and light hood, the tool will change how and when Canadian apple growers harvest their fruit. Learn in this video how the taste of science is sweet. For an accessible version, please visit: http://www.agr.gc.ca/eng?id=1423488316327
Views: 2747 AgricultureCanadaEng
Opensource spectrophotometer to check water quality
 
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After working on our swiss problems ... measuring the Patent Blue V in candies ... we are confronted with the Colombian problems, checking the water quality ... https://github.com/open-spectro/simple-spectro
Views: 84 Hackuarium
NanoDrop One Spectrophotometer Live Demo | ASHG 2015
 
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Voula Kodoyianni gives a hands-on demonstration of the NanoDrop One UV-Vis Spectrophotometer at the 2015 American Society of Human Genetics annual meeting. The NanoDrop One quantifies and qualifies DNA, RNA, and protein samples with only 1-2 µL and provides full-spectral data in seconds. Learn more at http://thermoscientific.com/nanodrop
A technique for characterizing organic matter associated with produced waters from oil extraction
 
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Presented by John Scott - Illinois Sustainable Technology Center Unconventional sources of oil and gas are becoming increasingly important in meeting US energy demands. Horizontal drilling and hydraulic fracturing of shales in locations such as Pennsylvania are commonplace in response to the need for domestically sourced energy. Although development of these new technologies has increased the domestic production of gas and oil, there have been many concerns relating to contamination of surface water, groundwater and drinking water supplies. Fluorescence excitation-emission spectroscopy is a rapid and inexpensive technique that could be utilized as a screening tool for water samples suspected of contamination by hydraulic fracturing operations. In conjunction with computational analysis of the fluorescence data using techniques such as parallel factor analysis (PARAFAC), questions such as "Has there been contamination caused by fracking" can be answered rapidly and inexpensively. Chemical additives used in fracturing fluids and chemical contaminates in produced waters, such as polyaromatic hydrocarbons (PAHs), could be used as a surrogate to determine if contamination of surface waters, groundwaters, and drinking waters was due to hydraulic fracturing operations in nearby locations. Methods developed in this project could aid in water quality monitoring and help guide decisions regarding further testing of water samples suspected of contamination.
Allergen testing by LC-MS/MS - Food & Enviro Summer Webinar Series
 
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This webinar highlights how LC-MS/MS can effectively be used to screen for and potentially quantify multiple allergens, including milk, egg, gluten, mustard, and others, in food samples. Steve Lock from AB SCIEX is the presenter.
Views: 535 SCIEX
OD Reading
 
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Views: 108 Brenden ONeill
Provost's Lecture Series: Dennis Slice
 
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In “An Unexpected Journey: A Curious Career in Shape Analysis,” Dr. Slice '93 discusses the developments in shape analysis during his graduate career, the people who influenced him, and his post-graduate work in software and methodological developments and applications. Dr. Slice also receives The Rohlf Medal for Excellence in Morphometric Methods and Applications.
Life Technologies Corp. v. Promega Corp.: Oral Argument - December 06, 2016
 
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Facts: Promega Corporation owned four patents and was the exclusive licensee of another one for technology used in kits that can conduct genetic testing. The kits are usually used for the purposes of identifying forensic or paternity matches. In 2010, Promega sued Life Technologies Corporation (LifeTech) for infringing on the patents in question, and LifeTech filed counterclaims that argued that the asserted claims of the patents were invalid. The district court determined that LifeTech had directly infringed on the patents and the case proceeded to damages. During the damages phase, there was a dispute about whether or not Promega had met its burden to prove that it was eligible for damages based on its worldwide sales. The jury determined that Promega was eligible for the worldwide damages, but the district court granted LifeTech’s motion to vacate the judgment because it determined that, as a matter of law, Promega had failed to present sufficient evidence to sustain that jury verdict. The U.S. Court of Appeals for the Federal Circuit reversed and determined that there was substantial evidence that LifeTech was liable for worldwide damages. Question: Does supplying a single component of a multi-component invention from the United States for sale abroad expose the manufacturer to liability for infringement based on worldwide sales? For more information about this case see: https://www.oyez.org/cases/2016/14-1538 Section 1: 00:00:05 Section 2: 00:15:41 Section 3: 00:26:06 Section 4: 00:56:36 PuppyJusticeAutomated videos are created by a program written by Adam Schwalm. This program is available on github here: https://github.com/ALSchwalm/PuppyJusticeAutomated The audio and transcript used in this video is provided by the Chicago-Kent College of Law under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License. See this link for details: https://creativecommons.org/licenses/by-nc/4.0/
List of IEC standards | Wikipedia audio article
 
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This is an audio version of the Wikipedia Article: List of IEC standards Listening is a more natural way of learning, when compared to reading. Written language only began at around 3200 BC, but spoken language has existed long ago. Learning by listening is a great way to: - increases imagination and understanding - improves your listening skills - improves your own spoken accent - learn while on the move - reduce eye strain Now learn the vast amount of general knowledge available on Wikipedia through audio (audio article). You could even learn subconsciously by playing the audio while you are sleeping! If you are planning to listen a lot, you could try using a bone conduction headphone, or a standard speaker instead of an earphone. You can find other Wikipedia audio articles too at: https://www.youtube.com/channel/UCuKfABj2eGyjH3ntPxp4YeQ You can upload your own Wikipedia articles through: https://github.com/nodef/wikipedia-tts "The only true wisdom is in knowing you know nothing." - Socrates SUMMARY ======= This is an incomplete list of standards published by the International Electrotechnical Commission (IEC). The numbers of older IEC standards were converted in 1997 by adding 60000; for example IEC 27 became IEC 60027. IEC standards often have multiple sub-part documents; only the main title for the standard is listed here. IEC 60027 Letter symbols to be used in electrical technology IEC 60028 International standard of resistance for copper IEC 60034 Rotating electrical machines IEC 60038 IEC Standard Voltages IEC 60041 Field acceptance tests to determine the hydraulic performance of hydraulic turbines, storage pumps and pump-turbines IEC 60044 Instrument transformers IEC 60045 Steam turbines IEC 60050 International Electrotechnical Vocabulary IEC 60051 Direct acting indicating analogue electrical measuring instruments and their accessories IEC 60052 Voltage measurement by means of standard air gaps IEC 60055 Paper-insulated metal-sheathed cables for rated voltages up to 18/30 kV (with copper or aluminium conductors and excluding gas-pressure and oil-filled cables) IEC 60059 IEC standard current ratings IEC 60060 High-voltage test techniques IEC 60061 Lamp caps and holders together with gauges for the control of interchangeability and safety IEC 60062 Marking codes for resistors and capacitors IEC 60063 Preferred number series for resistors and capacitors IEC 60064 Tungsten filament lamps for domestic and similar general lighting purposes – Performance requirements IEC 60065 Audio, video and similar electronic apparatus – Safety requirements IEC 60067 Dimensions of electronic tubes and valves IEC 60068 Environmental testing IEC 60071 Insulation co-ordination IEC 60072 Dimensions and output series for rotating electrical machines IEC 60073 Basic and safety principles for man-machine interface, marking and identification – Coding principles for indicators and actuators IEC 60076 Power transformers IEC 60077 Railway applications – Electric equipment for rolling stock IEC 60079 Explosive atmospheres IEC 60081 Double-capped fluorescent lamps – Performance specifications IEC 60083 Plugs and socket-outlets for domestic and similar general use standardized in member countries of IEC IEC 60085 Electrical insulation – Thermal evaluation and designation IEC 60086 Primary batteries; IEC 60092 Electrical installations in ships IEC 60094 Magnetic tape sound recording and reproducing systems IEC 60095 Lead-acid starter batteries IEC 60096 Radio frequency cables IEC 60098 Rumble measurement on Vinyl Disc Turntables IEC 60099 Surge arresters IEC 60100 Methods for the measurement of direct inter-electrode capacitance of electronic tubes and valves IEC 60104 Aluminium-magnesium-silicon alloy wire for overhead line conductors IEC 60105 Recommendation for commercial-purity aluminium busbar material IEC 60107 Methods of measurement on receivers for television broadcast transmissions IEC 60110 Power capacitors for induction heating installations IEC 60112 Method for the determination of the proof and the comparative tracking indices of solid insulating materials IEC 60114 Recommendation for heat-treated aluminium alloy busbar material of the aluminium-magnesium-silicon type IEC 60115 Fixed resistors for use in electronic equipment IEC 60118 Electroacoustics – Hearing aids IEC 60119 The Electrical Performance of Semiconductor Rectifiers (Metal Rectifiers) (Withdrawn) IEC 60120 Dimensions of ball and socket couplings of string insulator units IEC 60121 Recommendation for commercial annealed aluminium electrical conductor wire IEC 60122 Quartz crystal units of assessed quality IEC 60127 Miniature fuses IEC 60130 Connectors for frequencies below 3 MHz IEC 60134 Rating systems for electronic tubes and valves and analogous semiconductor devices IEC 60135 Numbering of electrodes and designation of units in electronic tubes and valves IEC 60136 Dimensions of brushes and brush ...
Views: 145 wikipedia tts
List of International Electrotechnical Commission standards | Wikipedia audio article
 
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This is an audio version of the Wikipedia Article: List of International Electrotechnical Commission standards Listening is a more natural way of learning, when compared to reading. Written language only began at around 3200 BC, but spoken language has existed long ago. Learning by listening is a great way to: - increases imagination and understanding - improves your listening skills - improves your own spoken accent - learn while on the move - reduce eye strain Now learn the vast amount of general knowledge available on Wikipedia through audio (audio article). You could even learn subconsciously by playing the audio while you are sleeping! If you are planning to listen a lot, you could try using a bone conduction headphone, or a standard speaker instead of an earphone. You can find other Wikipedia audio articles too at: https://www.youtube.com/channel/UCuKfABj2eGyjH3ntPxp4YeQ You can upload your own Wikipedia articles through: https://github.com/nodef/wikipedia-tts "The only true wisdom is in knowing you know nothing." - Socrates SUMMARY ======= This is an incomplete list of standards published by the International Electrotechnical Commission (IEC). The numbers of older IEC standards were converted in 1997 by adding 60000; for example IEC 27 became IEC 60027. IEC standards often have multiple sub-part documents; only the main title for the standard is listed here. IEC 60027 Letter symbols to be used in electrical technology IEC 60028 International standard of resistance for copper IEC 60034 Rotating electrical machines IEC 60038 IEC Standard Voltages IEC 60041 Field acceptance tests to determine the hydraulic performance of hydraulic turbines, storage pumps and pump-turbines IEC 60044 Instrument transformers IEC 60045 Steam turbines IEC 60050 International Electrotechnical Vocabulary IEC 60051 Direct acting indicating analogue electrical measuring instruments and their accessories IEC 60052 Voltage measurement by means of standard air gaps IEC 60055 Paper-insulated metal-sheathed cables for rated voltages up to 18/30 kV (with copper or aluminium conductors and excluding gas-pressure and oil-filled cables) IEC 60059 IEC standard current ratings IEC 60060 High-voltage test techniques IEC 60061 Lamp caps and holders together with gauges for the control of interchangeability and safety IEC 60062 Marking codes for resistors and capacitors IEC 60063 Preferred number series for resistors and capacitors IEC 60064 Tungsten filament lamps for domestic and similar general lighting purposes – Performance requirements IEC 60065 Audio, video and similar electronic apparatus – Safety requirements IEC 60067 Dimensions of electronic tubes and valves IEC 60068 Environmental testing IEC 60071 Insulation co-ordination IEC 60072 Dimensions and output series for rotating electrical machines IEC 60073 Basic and safety principles for man-machine interface, marking and identification – Coding principles for indicators and actuators IEC 60076 Power transformers IEC 60077 Railway applications – Electric equipment for rolling stock IEC 60079 Explosive atmospheres IEC 60081 Double-capped fluorescent lamps – Performance specifications IEC 60083 Plugs and socket-outlets for domestic and similar general use standardized in member countries of IEC IEC 60085 Electrical insulation – Thermal evaluation and designation IEC 60086 Primary batteries; IEC 60092 Electrical installations in ships IEC 60094 Magnetic tape sound recording and reproducing systems IEC 60095 Lead-acid starter batteries IEC 60096 Radio frequency cables IEC 60098 Rumble measurement on Vinyl Disc Turntables IEC 60099 Surge arresters IEC 60100 Methods for the measurement of direct inter-electrode capacitance of electronic tubes and valves IEC 60104 Aluminium-magnesium-silicon alloy wire for overhead line conductors IEC 60105 Recommendation for commercial-purity aluminium busbar material IEC 60107 Methods of measurement on receivers for television broadcast transmissions IEC 60110 Power capacitors for induction heating installations IEC 60112 Method for the determination of the proof and the comparative tracking indices of solid insulating materials IEC 60114 Recommendation for heat-treated aluminium alloy busbar material of the aluminium-magnesium-silicon type IEC 60115 Fixed resistors for use in electronic equipment IEC 60118 Electroacoustics – Hearing aids IEC 60119 The Electrical Performance of Semiconductor Rectifiers (Metal Rectifiers) (Withdrawn) IEC 60120 Dimensions of ball and socket couplings of string insulator units IEC 60121 Recommendation for commercial annealed aluminium electrical conductor wire IEC 60122 Quartz crystal units of assessed quality IEC 60127 Miniature fuses IEC 60130 Connectors for frequencies below 3 MHz IEC 60134 Rating systems for electronic tubes and valves and analogous semiconductor devices IEC 60135 Numbering of electrodes and designation of units in electronic tubes and valves IEC ...
Views: 69 wikipedia tts