Search results “Improved fluorometric method for determining selenium testing”
Qubit® 2.0 Fluorometer in Action
To Learn More visit, http://www.lifetechnologies.com/qubit The Qubit® 2.0 Fluorometer quantitates DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity. It is designed for molecular biology labs that work with: • Precious samples that are rare or difficult to process • Applications requiring precise measurement such as real-time PCR The Qubit® 2.0 Fluorometer utilizes specifically designed fluorometric technology using Molecular Probes® dyes. These fluorescent dyes emit signals ONLY when bound to specific target molecules, even in the presence of free nucleotides or degraded nucleic acids. Qubit® fluorometric quantitation provides the most specific and sensitive DNA and RNA quantitation available, even at low concentrations. Transcript Dr. Amr Abid: It's hard to believe how something so simple, so light can deliver such performance. That is exactly what Qubit 2.0 does. Ashleigh Young: Qubit was a real revolution when we launched version 1.0 in 2006. We learned so much from the scientists. We listened to their suggestions and we brought them into version 2.0. With Qubit 2.0, you don't need to change yourself to fit the device. It fits you. Dr. Amr Abid: Qubit 2.0 is simply the most specific and sensitive device amongst all nucleic acid and protein quantification devices. Voice: Much time, effort, and research money is invested to obtain precious nucleic acid and protein samples. We developed Qubit 2.0 because we believe that knowing precisely and accurately the quantity of DNA, RNA or protein is so critical to the success of the downstream applications like sequencing, PCR, RT-PCR, transfection and immunoassays. Dr. Amr Abid: The device provides trust and confidence ensuring downstream applications don't suffer from inaccurate quantification. Voice: It's no surprise that these reagents and devices are now recommended by leading journals and institutions as the best for quantifying RNA or DNA in applications like q-PCR, RT-PCR and sequencing. Qubit 2.0's specificity is due to the use of different fluorophores for DNA, RNA, single-stranded DNA and protein. UV quantification method simply cannot tell or confirm if what you have in the tube is RNA, DNA, nucleotide or a mix. Basic Qubit protocol is simple and shouldn't take more than two minutes. The first step is to prepare the working solution. Use 200 microliters of buffer for each tube. Use 1 microliter of dye for each tube. Because we have five tubes in our DNA quantification assays, we will need 5 microliters of dye and 1 mL of the buffer. Mix the dye and the buffer in 1.5 mL microtubes. Now we're ready to add the standards and the DNA sample. Use thin-wall PCR tubes. For DNA quantification assay, we need two standards. The total volume in each tube should be 200 microliters. Add 190 microliters of working solution prepared previously to each tube. Add 10 microliters of the standard one in the first tube and 10 microliters of standard two in the second tube. For the DNA sample, we can use between 1 to 20 microliters of each DNA solution to an assay tube. In this case, we add 198 microliters of the working solution prepared previously in our three DNA tubes. In each of the three DNA tubes, we add 2 microliters of DNA sample and mix. We incubate the five tubes at room temperature for two minutes. We now have our two standards and three DNA samples. We're ready to measure them in the Qubit 2.0 Fluorometer. On the homescreen, you have a choice for DNA, RNA, or protein quantification. Qubit 2.0 Fluorometer has a touch screen. To choose DNA, just touch the appropriate area. You will have a choice between different ways to quantify DNA: double-stranded DNA in high-sensitive mode, double-stranded DNA in broad range mode or single-stranded DNA. In our case, we will use the broad range mode. Standard screen is automatically displayed. Insert standard number one in the sample chamber and press "Read." When the reading is done, you'll be prompted to insert standard number two and press "Read." Qubit 2.0 Fluorometer is very simple and intuitive. It will guide the user through all the measurement steps. Ashleigh Young: The Qubit 2.0 is not just a new product. It's way more than that and yet thousands and thousands of users will instantly know how to use it. Performance with simplicity. This is the very DNA of Life Technologies. Voice: Qubit fluorometric quantitation: sensitive, specific, affordable. Simply better than [using].
Using Standard Curve to Estimate DNA Quantity - Forensic Focus #4
Submit your questions at http://www.thermofisher.com/forensicfocus Everyone wants to know how much DNA is in their extract, but then they ask: how can I tell if my estimate is accurate? The standard curve holds the answers. A standard curve is a tool that allows us to estimate the DNA concentration of unknown samples by comparing them to standards with known DNA concentrations. In this example, the standards consist of a 10-fold dilution series ranging from 50 ng/ul down to 5 pg/ul. During each PCR cycle, the amount of fluorescent signal for each standard in the dilution seies is measured. When the fluorescent signal crosses the detection threshold the cycle number is recorded as a Ct value, or threshold cycle value. The Ct value is what ultimately is used to create the standard curve. The Ct values are inversely proportional to the concentration of DNA in the standards. The high-concentration, 50 ng/ul standard will cross the detection threshold first, generating a “low” Ct. The low-concentration, 5 pg/ul standard will take many more cycles to cross the same threshold - and therefore the Ct will be higher. The Ct values for each dilution of the standard curve are plotted on a graph, and the software generates a regression line that fits the data. Because the standards are 10-fold dilutions, we expect the change in Ct from one standard to the next to be uniform. An uneven distribution of Ct values might indicate that the dilution series was not accurately pipetted. Let’s take a look at the standard curve for a specific DNA target, the small autosomal target. The X axis is the log of the known standard concentrations. The Y axis is the Ct value of each standard. Now, Do you see the quality metrics at the bottom of the screen? Let’s review Slope, Y intercept, and R2.? The slope measures the efficiency of the PCR reaction. In a perfect world, a slope of -3.3 indicates that the PCR reaction is 100% efficient; the target DNA is doubled each cycle. Two copies become four; four become eight; and so on. The Y intercept is the expected Ct value for a 1ng/ul sample. The R2 value measures how well the regression line fits the data points. A line that fits the data points perfectly has an R2 of 1. If your data points are scattered, the R2 value for the line will be lower. The Ct values of your standards affect the slope, the Y intercept, and the R2 value. It is very important to prepare the standard dilution series carefully to ensure consistent and accurate results! Running the standards in duplicate can help ensure you have a high quality standard curve. Once your standard curve passes the metrics test, it can be used to evaluate an unknown sample! The Ct value of the unknown sample is measured, and compared to the standard curve to estimate the DNA concentration of the unknown sample. Couldn’t be simpler! That’s it for today. If you have other questions, just click on the link below. And don’t forget- when in doubt, refer Back to the Bases!
Residual DNA Analysis Workflow
Learn more at http://lifetechnologies.com/resdnaseq This video contains Tips and Tricks for residual DNA quantitation using the resDNASEQ® Residual DNA Quantitation System, including PrepSEQ® Express Sample Preparation kits, the AutoMate Express™ Nucleic Acid Extraction System, and the Applied Biosystems® 7500 FAST Real-Time PCR System.
Sweet Science: A New Device for Checking Apple Ripeness
Agriculture and Agri-Food scientists in Summerland, B.C. and Kentville, N.S., are working to give apple producers cutting edge technology to ensure market success. They are developing data protocols for the DA Meter, a special tool that helps fruit producers to determine optimal ripeness of their crop. When it first came on the market, the DA Meter was not well adapted to reading apple ripeness. With the new protocols and light hood, the tool will change how and when Canadian apple growers harvest their fruit. Learn in this video how the taste of science is sweet. For an accessible version, please visit: http://www.agr.gc.ca/eng?id=1423488316327
Views: 2301 AgricultureCanadaEng
Tutorial: Infinite 200 PRO NanoQuant Plate™ for DNA measurements
The patented NanoQuant Plate's quartz optic guarantees superior performance and a high rate of reproducibility for up to 16 samples. Designed specifically for use with the Infinite 200 NanoQuant, this powerful tool is perfect for DNA/RNA quantification and quality control, providing reliable absorbance readings from as little as 2 μl of sample, and can detect DNA concentrations as low as 1 ng/μl. Key applications • DNA/RNA quantification during sample preparation for PCR-based assays, such as in research, genetics, forensics and blood banking laboratories • Measuring the labeling efficiency of dye-labeled samples, such as for FISH- and microarray-based experiments The flexible i-control software allows creation of application-oriented measurement scripts using its drag-and-drop interface for effortless set-up of new protocols, which can be saved on the system for future use. Online data presentation provides immediate review and analysis of your measurement data, with enhanced data management and straightforward export of data to Windows®-compatible formats (e.g. Excel®).
Views: 2513 Tecan
NanoDrop One Spectrophotometer Live Demo | ASHG 2015
Voula Kodoyianni gives a hands-on demonstration of the NanoDrop One UV-Vis Spectrophotometer at the 2015 American Society of Human Genetics annual meeting. The NanoDrop One quantifies and qualifies DNA, RNA, and protein samples with only 1-2 µL and provides full-spectral data in seconds. Learn more at http://thermoscientific.com/nanodrop
Provost's Lecture Series: Dennis Slice
In “An Unexpected Journey: A Curious Career in Shape Analysis,” Dr. Slice '93 discusses the developments in shape analysis during his graduate career, the people who influenced him, and his post-graduate work in software and methodological developments and applications. Dr. Slice also receives The Rohlf Medal for Excellence in Morphometric Methods and Applications.
Custom Assay Development Services by Life Technologies
Learn more at http://www.lifetechnologies.com/custom When an "off-the-shelf" assay does not provide the right fit for your studies, the Custom Biology Services team can craft an assay that helps solve your biological problems and meets your unique specifications, timelines and budget. Choose from custom biochemical, cell-based and TaqMan® protein assays; and collaborate with our Custom Biology team of scientists who are acutely familiar with the full range of Life Technologies assay formats. Then utilize our SelectScreen® services to seamlessly transition into screening your custom-made assay once the assay is developed. You know our customers really are the experts in the biology that they're studying. But as a tool provider, we have an intimate familiarity with the technology that can help our customers solve a biological problem. Some of the services that our custom biology team can offer to our customers include Custom Cellular and Biochemical Assay Development, SRNA Screening, TaqMan® Protein Assay Development as well as Cryopreservation services. Additionally, we can take Cell-based assays or stem cell-based assays and configure not just large scale provisioning these cells but we can transfer them directly into a screening operation and seamlessly move our customers from an assay-development paradigm into more of an operational screening exercise. Working with scientists from other companies is exciting because as scientists, we always want to keep our fingers on the pulse of the research community and working on custom projects is a great way to do that. On a personal level, it's really fulfilling. There's nothing better than hearing a customer say at the end of a project, "We're excited about what you've done. We're excited to get the material in. We're happy with the service and we want to continue this relationship and bring you more work in the future." Learn more at http://www.lifetechnologies.com/custom
On site detection of fungal spores in air
Rapid, on-site detection and quantification of fungal spores in greenhouses. Within 30 minutes the spore-load in the greenhouse is given. Much faster than standard plate incubation. No reduction in sensitivity or reliability. No concessions to make. Simply faster. This tool enables descision making on the spot instead of days after.
Views: 1966 InnosieveDiagnostics
Molecular-scale dynamics of light-induced spin cross-over in a two-dimensional layer
Molecular-scale dynamics of light-induced spin cross-over in a two-dimensional layer. Kaushik Bairagi et al (2016), Nature Communications http://dx.doi.org/10.1038/ncomms12212 Spin cross-over molecules show the unique ability to switch between two spin states when submitted to external stimuli such as temperature, light or voltage. If controlled at the molecular scale, such switches would be of great interest for the development of genuine molecular devices in spintronics, sensing and for nanomechanics. Unfortunately, up to now, little is known on the behaviour of spin cross-over molecules organized in two dimensions and their ability to show cooperative transformation. Here we demonstrate that a combination of scanning tunnelling microscopy measurements and ab initio calculations allows discriminating unambiguously between both states by local vibrational spectroscopy. We also show that a single layer of spin cross-over molecules in contact with a metallic surface displays light-induced collective processes between two ordered mixed spin-state phases with two distinct timescale dynamics. These results open a way to molecular scale control of two-dimensional spin cross-over layers.
Views: 52 ScienceVio
Life Technologies Corp. v. Promega Corp.: Oral Argument - December 06, 2016
Facts: Promega Corporation owned four patents and was the exclusive licensee of another one for technology used in kits that can conduct genetic testing. The kits are usually used for the purposes of identifying forensic or paternity matches. In 2010, Promega sued Life Technologies Corporation (LifeTech) for infringing on the patents in question, and LifeTech filed counterclaims that argued that the asserted claims of the patents were invalid. The district court determined that LifeTech had directly infringed on the patents and the case proceeded to damages. During the damages phase, there was a dispute about whether or not Promega had met its burden to prove that it was eligible for damages based on its worldwide sales. The jury determined that Promega was eligible for the worldwide damages, but the district court granted LifeTech’s motion to vacate the judgment because it determined that, as a matter of law, Promega had failed to present sufficient evidence to sustain that jury verdict. The U.S. Court of Appeals for the Federal Circuit reversed and determined that there was substantial evidence that LifeTech was liable for worldwide damages. Question: Does supplying a single component of a multi-component invention from the United States for sale abroad expose the manufacturer to liability for infringement based on worldwide sales? For more information about this case see: https://www.oyez.org/cases/2016/14-1538 Section 1: 00:00:05 Section 2: 00:15:41 Section 3: 00:26:06 Section 4: 00:56:36 PuppyJusticeAutomated videos are created by a program written by Adam Schwalm. This program is available on github here: https://github.com/ALSchwalm/PuppyJusticeAutomated The audio and transcript used in this video is provided by the Chicago-Kent College of Law under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License. See this link for details: https://creativecommons.org/licenses/by-nc/4.0/
Thermo Scientific NanoDrop Lite - Simon Nunn
http://www.thermoscientific.com/nanodrop - Speaker: Simon Nunn Simon Nunn introduces the Thermo Scientific NanoDrop Lite , a productivity breakthrough for nucleic acid and purified protein measurements...and small enough to fit in a drawer. It's a compact, personal UV-Vis microvolume spectrophotometer that complements full-featured Thermo Scientific NanoDrop™ instruments. Learn how this new solution eliminates wasteful dilutions of test sample, offers easy-to-use pre-programmed methods and reduces use of expensive consumables. Its compact, yet powerful with accurate reproducible measurements and offers an optional docking printer sold separately.